Generator
Part:BBa_K1758201:Design
Designed by: Team Bielefeld-CeBiTec 2015 Group: iGEM15_Bielefeld-CeBiTec (2015-08-28)
His-tagged lac repressor under the control of the T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a composite part which enables a high protein expression and an easy purification of lacI.
Source
Parts from the parts registry are combined and a His-Tag is added through PCR and Gibson Assembly.